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mouse anti ova ige standards  (Bio-Rad)


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    Bio-Rad mouse anti ova ige standards
    Mouse Anti Ova Ige Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+ova+ige+standards/Mouse+anti+Ovalbumin/us11826422-666-22-26
    Average 94 stars, based on 40 article reviews
    mouse anti ova ige standards - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Methods and compositions for adaptive immune modulation
    Article Snippet: Female BALB/c mice (6-10 weeks of age) were purchased from Charles River, Wilmington, Mass., and housed five mice per cage in a 12/12 h light/dark cycle with food and water available ad libitum. .. For OVA IgE ELISAs, wells were coated with 2 μg/mL goat anti-mouse IgE (AbD Serotec, STAR166) overnight at 4° C. After washing, mouse anti-OVA IgE standards (AbD Serotec, clone 2C6) or diluted mouse serum samples (usually 1:50) were incubated for 1 h at 37° C. After washing, 10 μg/mL biotin-conjugated OVA (EZ-Link Sulfo-NHS-LC-Biotin, Thermo Scientific) were incubated for 1 h at 37° C. Wells were washed as indicated and 1:5,000 Neutravidin-HRP (Thermo Scientific) was incubated for 30 minutes at 37° C., followed by two more washes with TBS before developing the samples. .. The same protocol was followed for determining total IgE using the following amounts of reagents: 2 μg/mL goat anti-mouse IgE (AbD Serotec, STAR166), 2 μg/mL Biotin Rat Anti-Mouse IgE (BD Biosciences, clone R35-118).



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    Effect of feeding mice for 7 weeks either normal diet or high protein diet on serum levels of (A) OVA-specific IgE (B) OVA-specific IgG1 and (C) OVA-specific IgG2a from OVA-sensitized mice (n = 11 in each group) after OVA intranasal challenge. The high protein diet group showed higher levels of both OVA-specific IgE and IgG1compared to the normal diet group. ns: not significant, CI: confidence interval. IQR: interquartile range.

    Journal: The World Allergy Organization Journal

    Article Title: High protein diet increases the risk of allergic sensitization but not asthma in mice through modulation of the cytokine milieu toward Th2 bias ☆

    doi: 10.1016/j.waojou.2025.101031

    Figure Lengend Snippet: Effect of feeding mice for 7 weeks either normal diet or high protein diet on serum levels of (A) OVA-specific IgE (B) OVA-specific IgG1 and (C) OVA-specific IgG2a from OVA-sensitized mice (n = 11 in each group) after OVA intranasal challenge. The high protein diet group showed higher levels of both OVA-specific IgE and IgG1compared to the normal diet group. ns: not significant, CI: confidence interval. IQR: interquartile range.

    Article Snippet: After washing the wells, blocking buffer (0.5% gelatin in wash buffer or 3% Rat serum in wash buffer) was added and the plates incubated for 2 h. Appropriate reference standard OVA-IgE (Chondrex #3006) or OVA-IgG1 (Chondrex # 7093) or OVA-IgG2a (Chondrex # 7095) and diluted serum samples were then added to the wells and incubated for 3 h. This was followed by incubation with OVA-Biotin for 1 h and with streptavidin-HRP for 30 min. Tetramethylbenzidine substrate was added and the enzyme-substrate reaction stopped after 15 min, and the optical density measured at 450 nm.

    Techniques: